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ctt-journal > Volkova 2 et al. (Abstract)

Volkova 2 et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract41
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010

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Molecular-genetic SSP HLA-typing of samples of cord blood for the public register of cord blood donors

Olga Ya. Volkova2,3, Elena A. Kotelevskaya3, Alexandrina S. Khrupina3, Andrei V. Novitskyi1, Alexander B. Smolyaninov3

1Military Medical Academy, Saint-Petersburg, Russia; 2City station of blood transfusion, Saint-Petersburg, Russia; 3“Stem cell bank Pokrovsky” ltd., Saint-Petersburg, Russia

Correspondence: Olga Ya. Volkova, “Stem cell bank Pokrovsky” ltd., 85, Bolshoi prospect V.O., 199106, Saint-Petersburg, Russia, E-mail: stemcellbank@spam is badinbox.ru

Abstract

Umbilical cord blood (CB) contains hematopoietic stem cells (HSC) and in certain cases can be used as an alternative to bone marrow transplantation. Currently the “Pokrovsky” Stem Cell Bank organizes the first storage of cord blood samples intended for public use — the register of cord blood samples. This can help to solve the problem of HSC unit deficiency in Russia. The most important factor in effective transplantation is the degree of HLA matching. The aim of this work is HLA-typing with low-resolution of each cord blood unit.

Currently 250 samples of cord blood have been analyzed using a polymerase chain reaction (PCR) with sequence specific primers (SSP). DNA was isolated from 0.7 ml of whole blood with a Protrans DNA Box 500 DNA extraction column (Protrans, Germany). HLA-A, HLA-B and HLA-DRB1 loci were analyzed using a HLA-A*,-B*,-DRB1* Protrans cyclerplate system. (Protrans, Germany) and Biotest HLA SSP Kits (Biotest Medical Diagnostics, Germany), according to the manufacturer’s instructions.

It was identified that the most frequent alleles are: HLA-A*02: 28%, *03: 15%, and *24: 13%; HLA-B*35: 12.8%, *07: 10%, and *08: 7%, and HLA-DRB1*07: 16.5%, *15: 14%, and *01: 13.4%.

The results of HLA-typing with low resolution could be applied to primary selection of donors when there is a need of HSC transplantation. Also it significantly simplifies the typing operation with high resolution. Advantages of the own HLA-identified register of cord blood samples in Russia are accessibility of cord blood samples and decreasing their cost. But the major advantage is a higher chance of HLA-matching in the same population.

Keywords: hematopoietic stem cells, umbilical cord blood, cord blood public register, transplantation, HLA-typing

 

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