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ctt-journal > Volchkov et al. (Abstract)

Volchkov et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract04
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010

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Defining the best source of human multipotential mesenchymal stromal cells (MSC)

Stanislav E. Volchkov, Pavel V. Boriskin, Larisa M. Trusova, Andrey N. Toropovskiy, Olga V. Tyumina

The Clinical Center of Cellular Technologies, Samara, Russia

Correspondence: Stanislav E. Volchkov, The Clinical Center of Cellular Technologies, 159 Tashkentskaya street, 443095, Samara, Russia, E-mail: quality@spam is badcordbank.ru

Abstract

The aim of the current study is to define the best source of MSC from three easily available sources.

Materials and methods: We studied 26 cord blood units, 15 bone marrow samples, and 15 lipoaspirate samples. We investigated the proliferative potential, morphology changes, differentiation into mesenchymal lineages, phenotyping with a recommended panel, and cytokine synthesis. To estimate the ability for establishing a combined transplant we seeded cells on different types of matrixes, including lyophilized bone, titanium, nickel titanium, and nickel titanium with hydroxyapatite. To estimate MSC numbers on surfaces we used scanning electron microscopy. 

Results: We estimated that fat-derived cells have the lowest doubling time (0.0088/hr) and the highest CFU-F frequency (0.25% per mononuclear cells), leading to maximum yields of regenerative cells over a minimum amount of time. The cells from all three sources successfully differentiated into all three lineages. All cells were positive for mesenchymal and negative for hematopoietics markers. We didn’t find any statistically significant differences in cytokine expression within cells from various sources. All cells successfully established mixed transplants with matrixes. Our study shows that the cells from lipoaspirate are the best candidate for regenerative medicine due to their having identical properties with cells from other sources, accessibility to collect big volumes, and maximum yields at lowest culture time because of highest CFU-F frequency.

Discussion: To achieve a high amount of MSC over a short time and with low invasiveness it is better to choose lipoaspirate as the cell source.
 
Keywords: mesenchymal cells, bone marrow, cord blood, lipoaspirate, combined implants

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