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ctt-journal > Smirnova et al. (Abstract)

Smirnova et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract31
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010

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Experimental research: cellular therapy with adipose derived mesenchymal stem cells for fatty fabric osteoarthritis in large animals

Natalia V. Smirnova1,2, Polina Yu. Novikova1,2, Alexander B. Smolyaninov1

1“Stem cell bank Pokrovsky” ltd., Saint-Petersburg, Russia; 2Institute of Cytology of RAS, Saint-Petersburg, Russia

Correspondence: Natalia V. Smirnova, “Stem cell bank Pokrovsky” ltd., 85, Bolshoi prospect V.O., 199106, Saint-Petersburg, Russia, E-mail: nvsmirnoff@spam is badyandex.ru

Abstract

Osteochondral disorders with painful failure of the talocrural joint cause inconvenience for patients and decrease their vital activity. A joint cartilage defect causes pain and swelling in the lower limbs, regardless whether they are under load or at rest. A promising direction for repairing cartilage defects is the implantation of autologous adipose derived stem cells (ASCs). ASCs provide an abundant and easily accessible source of adult stem cells for use in such regenerative approaches.

For our research we used the case of severe ankle osteoarthritis in a dog. Adipose tissue was collected aseptically via surgical lipectomy. This protocol first describes the multi-stage isolation of ASCs. The cell culture conditions provided for ASC expansion provided a large number of cells. ASCs were injected directly into the affected joints both intra-articularly and intravenously, to induce a more global effect. Within 3 months we noticed increased joint flexibility and the ability to walk, and less pain in both osteoarthritic knees, leading to a decrease in pain medication.

ASCs can differentiate into many tissue types, induce repair, and stimulate regeneration. Through a variety of studies, ASCs have shown the capacity to be differentiated along mesodermal lineages including osteogenic, adipogenic, myogenic and chondrogenic lineages as well as ecto- & endodermal lineages. They can also “communicate” with the cells of their local environment through paracrine and autocrine modalities, creating the optimal environment for natural healing. Angiogenic cytokines such as vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) were reported as ASCs products, which are able to contribute to the ASCs’ angiogenic properties.

Keywords: adipose derived stem cells, osteoarthritis, regeneration

 

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