Shman et al. (Abstract)
Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract06
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported
Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010
Natural killer cell alloreactivity in allogeneic hematopoietic stem cell transplantations for children
Tatyana V. Shman, Katsiaryna P. Vashkevich, Alexandr A. Migas, Valery P. Savitski, Yuliya E. Mareika, Nina V. Minakovskaya
Belarusian Research Center for Pediatric Oncology and Hematology, Minsk, Belarus
Correspondence: Tatyana V. Shman, Belarusian Research Center for Pediatric Oncology and Hematology, Pos. Lesnoe-2, 223040, Minsk reg., Belarus, E-mail: t_shman@yahoo.com
Abstract
Objective: Donor natural killer (NK) cells can initiate alloreactions following allogeneic hematopoietic stem cell transplantations (alloHSCT), preventing GvHD and relapse recurrence.
Aim: Evaluation of the significance of NK alloreactivity in alloHSCT for children
Patients and methods: Inhibitory KIR (KIR2DL1, KIR2DL2/KIR2DL3, KIR3DL1) were genotyped and phenotyped for donor NK cells. The presence of ligands (HLA-С1, HLA-С2, HLA-Bw4) was estimated based on the HLA-I typing data. Donor-recipient pairs were divided into two groups depending on the presence (negative alloreactivity) or absence (positive alloreactivity) of HLA-I ligands for donor inhibitory KIR.
Results: Donor NK cells had alloreactivity in 34 out of 49 analyzed cases. In the early point after HSCT the significant decrease of activated T cells in peripheral blood was detected in the group of patients with alloreactivity. Moreover, for patients with non-malignant diseases (aplastic anemia, immunodeficiency), the rate of grade 2–4 acute GvHD was 18.2% in alloreactivity cases as compared with 75.0% in the group of patients without alloreactivity (р=0.04). However, for patients with malignant diseases (AML, ALL, MDS, CML) we did not find this type of difference in the rate of acute GvHD.
For patients with malignant diseases the tendency to decreased overall survival after HSCT in the negative alloreactivity group was observed, compared to that in the positive alloreactivity patients. In the non-malignant group of patients we found the opposite tendency.
Keywords: allogeneic hematopoietic stem cell transplantation, natural killer cells, killer immunoglobulin-like receptors (KIR)
