Novitsky et al. (Abstract)
Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract53
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported
Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010
Immunological monitoring in patients with lymphoproliferative disorders after autologous hematopoietic stem cell transplantation
Andrey V. Novitsky, Vladimir Y. Nikitin, Dmitriy А. Gornostayev, Аndrey М. Ivanov
Medical Military Academy, Saint-Petersburg, Russia
Correspondence: Andrey V. Novitsky, Dept. of Bone marrow transplantation of the Medical Military Academy, Lebedev str 6 A, 194044, Saint-Petersburg, Russia, E-mail: anov1@mail.ru
Abstract
Background. Hematopoietic stem cell transplantation (HSCT) is the therapy of choice in aggressive and treatment-resistant malignant lymphomas. The immunological reconstitution of lymphoma patients is problematic in the early period after auto-HSCT.
Materials and methods. Immunological monitoring of 4 patients with malignant lymphomas took place. Flow cytometric analysis was performed with a “Cytomics FC500”, before auto-PHSCT and after peripheral blood cell reconstitution.
Results. A complete response was achieved in all patients. After peripheral blood cell reconstitution, a Т-h cell immunodeficiency was detected, characterized by a significant decrease in T helper numbers and activated T-lymphocyte cytotoxic predominance, along with a rise in the common T-lymphocyte percentage, with a dramatic misbalance in the whole T-lymphocyte population. Besides this, a raised HLA-DR-positive T-lymphocyte level was detected. A marked reduction in the B-lymphocyte population and activated B-cells was found, and a number of humoral immunity indices such as serum IgА were also decreased.
Conclusion. In all auto-PHSCT patients, regardless of a disease stage and morphology, we observed the identical immunological disturbances alongside a complete response, indicating a secondary T-h cell mediated immune deficiency, with cytotoxic activated T-lymphocyte predominance in combination with В-cell immunodeficiency.
Keywords: immunological screening, auto-PSCT, immunodeficiency, immunity, lymphoproliferative disorders, lymphomas
