Lepik et al. (Abstract)
Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract65
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported
Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010
Platelet lysate as an alternative growth factor source for in vitro expansion of mesenchymal stem cells
Kirill V. Lepik, Olga V. Burdanova, Ildar M. Barkhatov, Boris V. Afanasyev
R.M. Gorbacheva Memorial Institute of Children Hematology and Transplantation, Pavlov State Medical University, Saint-Petersburg, Russia
Correspondence: Ildar M. Barkhatov, R.M. Gorbacheva Memorial Institute of Children Hematology and Transplantation, Pavlov State Medical University, 6/8, L. Tolstoy str., Saint-Petersburg, 197022, Russia, E-mail: i.barkhatov@gmail.com
Abstract
Background: Due to existing perspectives on the clinical usage of bone marrow mesenchymal stem cell (MSC) populations, the most current tasks are development of their isolation, expansion, and infusion methods. Presently, however, in most protocols for MSC expansion, the culture medium includes the addition of fetal cow serum (FCS), which is a potential source of xenogenic antigens as well infection contamination. Human platelet lysate (HPL) from platelet enriched plasma could be considered as an alternative to FCS.
Objectives: The aim of the study was the development of HPL extraction techniques, and evaluation of its biological activity and possible use as an alternative to FCS.
Methods: We investigated the proliferative activity of MSCs in primary culture and during long-term cultivation. The immunophenotype, gene expression profile, differentiation ability, and hematopoiesis support function were compared with FCS-supplemented and serum-free medium.
Results: In assessing MSCs' colony formation in primary culture the number of colony-forming units of fibroblasts (CFU-f) was higher when HPL was used in comparison with FCS. Differences in MSCs' proliferation capacity was not observed during long-term cultivation. A lower number of CD14+ cells and reduced level of NGF, osteopontin, ITGA7, and NTF3 gene expression was identified when HPL was used. The ability of MSCs' osteogenic differentiation was higher in HPL-supplemented medium.
Conclusion: During expansion of MSCs using HPL their differentiation ability was changed with accompanied gene expression profile changes and with proliferation ability that was comparable to FCS.
Keywords: mesenchymal stem cells, platelet lysate, fetal bovine serum
