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ctt-journal > Kuzmina Z.1 et al. (Abstract)

Kuzmina Z.1 et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract61
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010

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Significant differences in B-cell subpopulations and dysgammaglobulinemia characterize patients with chronic graft-versus-host disease

Zoya Kuzmina1, Hildegard T. Greinix1, Roman Weigl1, Ulrike Körmöczi2, Arno Rottal2, Sandra Eder1, Winfried F. Pickl2


1Department of Internal Medicine I, Bone Marrow Transplantation, Medical University of Vienna; 2Institute of Immunology, Medical University of Vienna, Austria

Correspondence: Zoya Kuzmina, Medical University of Vienna, Internal Medicine I, Bone Marrow Transplantation Department, Waehringer Guertel 18-20, 1090, Vienna, Austria, E-mail: zoya.kuzmina@spam is badmeduniwien.ac.at

Abstract

Manifestations of chronic graft-versus-host disease (cGVHD) can resemble those seen in immunodeficiency states and autoimmune disorders including, amongst others, hypogammaglobulinemia and the occurrence of autoantibodies.

Aim: We investigated circulating B lymphocyte subpopulations in cGVHD patient cohorts defined by serum immunoglobulin levels using multicolor flow cytometry, with the aim of characterizing novel biomarkers to predict their clinical course after allogeneic hematopoietic stem cell transplantation (HSCT).

Patients: Seventy-six patients with cGVHD were enrolled 42 (range, 4–171) months after HCT. Serum IgG-levels (hypogammaglobulinemia < 700 – 1600 mg/dl > hypergammaglobulinemia) were used to stratify groups of patients.

Results: Significantly more patients with hypergammaglobulinemia than those with hypogammaglobulinemia had detectable autoantibodies (72% vs. 18%, p=0.02). The hypogammaglobulinemia group presented with significantly diminished CD19+ B cell numbers (165 vs. 417 vs. 454 x 106/L, p<0.0001), and with concomitantly elevated immature CD19+CD21- (16.5%, 7.5% and 9.2%, p<0.01) B cell proportions when compared to the normo- and hypergammaglobulinemia group. Moreover, naïve (125 vs 342 x 106/L, p=0.04), class-switched (16 vs. 22 x 106/L, p=0.03) and non-class-switched B cells (4 vs. 11 x 106/L, p<0.03) were significantly lower in the hypogammaglobulinemia group compared to the control group. Transitional B cells were significantly elevated in all patients with cGVHD, and mainly in the hypogammaglobulinemia group (5.3% vs. 10.5%, p=0.02).

Conclusions: Our data indicates the different pathogenetic mechanisms of cGVHD leading to different clinical presentations.

Keywords: hematopoietic stem cell transplantation, chronic graft-versus-host disease, B-cells, biomarker

 

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