Ivanova et al. (Abstract)
Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract43
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported
Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010
The first experience of searching within local bone marrow registries and cord blood banks in the Russian Federation for donors for children with hematological and oncological disorders
Anna S. Ivanova1,2, Grigory A. Tsaur1,2, Tatyana A. Suslova3, Olga V. Yanina3, Andrey N. Тоropovskiy4, Olga V. Тyumina4, Lyudmila V. Еrochina5, Lyudmila N. Bubnova5, Yury G. Yoffe6, Vera М. Мinina7, Lyubov V. Кulagina7, Maria V. Yakovleva8, Yulia A. Yakovleva1,2, Tatyana O. Riger1,2, Alexander M. Popov1,2,9, Egor V. Shorikov1,2, Leonid I. Saveliev1,2,9, Larisa G. Fechina1,2
1Regional Children’s Hospital No. 1, Ekaterinburg, Russia; 2Institute of medical cell technologies, Ekaterinburg, Russia; 3Regional blood transfusion station, Chelyabinsk, Russian; 4Clinical center of cell technology, Samara, Russia; 5Russian research institute of hematology and transfusiology, St.Petersburg, Russia; 6Karelian registry of unrelated hematopoietic stem cell donors, Petrozavodsk, Russia; 7Regional blood transfusion station, Pervouralsk, Russia; 8Stem cell bank, Moscow, Russia; 9Ural State Medical Academy, Ekaterinburg, Russia
Correspondence: Anna S. Ivanova, Regional Children Hospital №1, Pediatric Oncology / Hematology Center, 32, S. Deryabina str., Ekaterinburg, 620149, Russia, E-mail: anna.ivanova1909@gmail.com
Abstract
Methods. From October 2006 to May 2010 146 molecular HLA-typings were performed in the Regional Children’s Hospital No. 1 for 72 patients and 74 potential related donors. Respectively, 142 and 24 samples were analyzed by PCR with sequence-specific primers and sequence-based HLA-typing. Both methods were applied in 20 cases.
Results. Among 84 examined siblings, 11 completely identical pairs, 39 haploidentical cases, and 23 mismatched cases were found. Forty-seven donor request searches were sent to local stem cell donor registries, and 11 to cord blood banks throughout the Russian Federation. Results are shown in the table below. Low-resolution DNA-typing identified 15 matched donors (9/10 and 10/10 matches). High-resolution DNA typing, performed at the Molecular biology laboratory of Regional Children Hospital No. 1, allowed us to detect 4 matched (10/10) unrelated donors, 22 HLA-identical siblings, and one unique case of a mother-daughter matched pair. In 9 detected matched pairs (5 related and 4 unrelated) DNA samples were sent for reference HLA-typing to an EFI-accredited HLA-laboratory (Austria). No discrepancies were found.
Conclusions. Our preliminary data shows that for wide-spread HLA-genotypes matched donors can be found even within relatively small local registries.
Local register | Number of requests | Results of low-resolution HLA-typing | |||||
|---|---|---|---|---|---|---|---|
10/10 matches | 9/10 matches | <9/10 matches | 6/6 matches | 5/6 matches | <5/6 matches | ||
Stem cell donor registries | |||||||
Stem cell donor registry, Chelyabinsk | 28 | 5 | 9 | 7 | – | – | – |
Russian Center of immunological tissue typing, St. Petersburg | 11 | – | – | – | – | – | 47 |
Regional laboratory of immunological tissue typing, Pervouralsk | 7 | 1 | – | – | – | – | 31 |
Karelian registry of unrelated hematopoietic stem cell donors, Petrozavodsk | – | – | – | – | – | – | 1 |
Cord blood banks | |||||||
Stem cell bank, Moscow | 4 | – | – | – | – | – | 8 |
Clinical center of cell technology, Samara | 7 | – | – | – | 2 | 36 | 84 |
Total | 58 | 6 | 9 | 7 | 2 | 36 | 170 |
Keywords: HLA-typing, stem cell transplantation, donor, pediatric oncology and hematology
