[English]  [Pусский]  [中文]  
 
ctt-journal > Druy et al. (Abstract)

Druy et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 9
doi: 10.3205/ctt-2010-No9-abstract45
© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "4th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2010

Contribute a comment

 

Evaluation of tyrosine hydroxilase (TH), GD2 and ELAVL4 expression detection for bone marrow (BM) involvement in neuroblastoma (NB) patients

Alexander E. Druy1,2, Grigory A. Tsaur1,3, Alexander M. Popov1,2,3, Egor V. Shorikov1,3, Leonid I. Saveliev1,2,3, Larisa G. Fechina1,3

1Regional Children Hospital No.1, Ekaterinburg, Russia; 2Ural state medical academy, Ekaterinburg, Russia; 3Institute of medical cell technologies, Ekaterinburg, Russia

Correspondence: Alexander E. Druy, Regional Children Hospital No.1, Pediatric Oncology / Hematology Center, 32, S. Deryabina str., Ekaterinburg, 620149, Russia, E-mail: 283850@spam is badgmail.com

Abstract

Introduction. Recently, it was identified that PHOX2B is presently the best single marker for PCR-based detection of BM lesion in NB patients (pts).

Aim. To study the applicability of TH, GD2, and ELAVL4 expression detection in comparison with PHOX2B for the assessment of BM involvement in NB patients.

Methods. Molecular marker expression was evaluated by real-time PCR in 183 BM samples obtained from 37 NB pts, in 16 BM samples from 16 patients without malignancies, and in 2 neuroblastoma cell lines (IMR-32, Kelly). The samples were considered as positive if detectable PHOX2B expression or tumor cells were present in the BM smears. The threshold level (TL) for each marker was calculated by ROC curve analysis. Diagnostic sensitivity (DS), specificity (Sp), positive and negative predictive values (PPV, NPV), and overall correct prediction (OCP) were calculated.

Results. PHOX2B and TH expression was not detected in normal BM. GD2 and ELAVL4 expression was revealed in 10 out of 16 normal BM samples. TH and PHOX2B ROC-curves did not differ (p=0.224). TH had high values of DS, Sp, PPV, NPV, and OCP, similar to those of PHOX2B. Results are shown in the table below. The diagnostic performance tests of GD2 and ELAVL4 expression detection were notably lower. We also tested a diagnostic approach where samples were defined as positive in the case of PHOX2B and/or TH expression above the TL. In comparison with PHOX2B, the addition of TH led to higher DS and NPV, but lower Sp and OCP.

Conclusions. PHOX2B and TH have similar diagnostic values for BM involvement assessment in NB pts. The simultaneous application of PHOX2B and TH does not bring significant benefits in comparison with PHOX2B alone. GD2 and ELAVL4 expression detection is less relevant for BM involvement assessment in NB patients.

DS

Sp

PPV

NPV

OCP

PHOX2B

0.960

1.000

1.000

0.977

0.985

TH

0.985

0.969

0.944

0.976

0.946

GD2

0.413

0.984

0.939

0.740

0.772

ELAVL4

0.573

0.961

0.896

0.792

0.817

PHOX2B and/or TH

0.973

0.969

0.948

0.984

0.970


Keywords:
neuroblastoma, bone marrow involvement, PHOX2B, tyrosine hydroxilase (TH), PCR

 

<-- Previous abstract        Contents        Next abstract -->

Contribute a comment

Top