[English]  [Pусский]  [中文]  
 
ctt-journal > Supilnikova et al. (Abstract)

Supilnikova et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract90

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

Contribute a comment

 

Laboratory screening of cord blood samples from the public donor registry

Olga V. Supilnikova, Irina I. Maslenikova, Alexander B. Smolyaninov

Research laboratory of cell technologies of the North-Western State Medical University named after I. I. Mechnikov, St. Petersburg, Russia; "Stem Cell Bank Pokrovsky", St. Petersburg, Russia

Correspondence: Alexander B. Smolyaninov, "Stem Cell Bank Pokrovsky", Bolshoi Prospect Vasilevski Ostrov 85, St. Petersburg, 199106, Russia, E-mail: stemcellbank@spam is badinbox.ru

Abstract

Purpose: To determine the presence of infectious agents in samples of umbilical cord blood from the public register of donors.

Methods:
To detect specific antibodies of infectious agents (Anti-HIV1 and 2, Anti-HTLV-I and -II, Anti-HBcor-Ag, Anti-HCV, Anti-CMV, Anti-Toxoplasma gondii, Anti-Tr. Pallidum) in cord blood plasma, the enzyme-linked immunosorbent assay (ELISA) method was used, and for detection of nucleic acids (HBV, HCV) a polymerase chain reaction (PCR) was used.

The results of these studies have shown that antibodies to HbcorAg, HCV, CMV, T. gondii, T. pallidum were detected in 3.8%, 1.6%, 53.5%, 3.3%, and 1.5% of samples respectively, and the simultaneous detection of antibodies to CMV and T.gondii was detected in 19.6% of cases. The lack of positive results in identifying HIV1 p24 antigen and antibodies to HIV1, 2 (ELISA), HBV and HCV (PCR) was due to the exclusion of mothers infected with HIV, HBV, and HCV. Nor antibodies to HTLV I and II were found in the samples. None of these markers were detected in 16.7% of cord blood samples. Thus, the share of discharged samples due to an infectious risk was 6.9%. A large number of samples containing antibodies to CMV, and T. gondii show a high occurrence of these infections in the population and isn’t an indication to discharge these samples.

Studies have shown that to reduce the percentage of discharged cord blood samples in the public register mandatory laboratory screening for Anti-HBcore should be included in the analysis of the mothers. The need to identify markers of infectious agents according to Order of 25.07.2003 No. 325 by the Ministry of Health is still in effect.

Keywords:
cord blood sample, infectious agents, enzyme immunoassay, polymerase chain reaction, antigens, antibodies