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ctt-journal > Sokolova et al. (Abstract)

Sokolova et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract07

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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The KIR profile of potential donors of the Republican Register of bone marrow / hematopoietic cell donors

Julia V. Sokolova, Ludmila N. Bubnova, Irina E. Pavlova, Stanislav S. Bessmeltsev

Russian Research Institute of Hematology and Transfusiology, St. Petersburg, Russia

Correspondence: Julia V. Sokolova, Russian Research Institute of Hematology and Transfusiology, 16, 2nd Sovietskaya str., St. Petersburg, 191024, Russia, E-mail: July_Sokolova@spam is badmail.ru, immgenSPB@spam is badgmail.com  

Abstract

Donor KIR genotype influences the outcome of hematopoietic cell transplantation: donor haplotype group B improves the therapeutic effect of transplantation in acute myeloid leukemia, whereas homozygosity for the allele-expressing single activating gene of AA genotype KIR2DS4 is regarded as a risk factor for acute graft-versus-host disease.

Purpose: Examine the distribution of KIR-gene and genotype in donors on the Republican Register.

Methods: KIR-genotyping of 100 potential donors was carried out using the commercial KIR Genotyping SSP Kit (Invitrogen, WI, USA).

Results: A total of 21 different KIR genotypes were found in the study group: AA genotype in 33%, and Bx in 67% of donors.

The frequencies of inhibitory KIRs were: 2DL1: 96%; 2DL2: 47%; 2DL3: 85%; 2DL5: 52%; and 3DL1: 96%. The frequencies of activating KIRs were: 2DS1: 40%; 2DS2: 47%; 2DS3: 39%; 2DS4: 96%; 2DS5: 23%; and 3DS1: 35%.

The occurrence of B-haplotype associated KIR-genes (2DL2, 2DS5, 3DS1) was substantially lower than in other Caucasoid populations. Among 33 donors with the AA-genotype, only one donor was homozygous for the allele 2DS4, encoding expressing receptors.

Conclusions: The frequency of KIR genes and genotypes in donors from the Northwest region of Russia shares several general features with other Caucasoid populations. The carriers of some B-haplotype associated activating KIR-genes were less frequent. Genotype AA with two expressing KIR2DS4 alleles, that is associated with the risk of complications in the transplantation, occurred in only 1% in the group under study.

Keywords: KIR genes, KIR genotype, hematopoietic cell transplantation, donor, Republican Register