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ctt-journal > Smirnova N.2 et al. (Abstract)

Smirnova N.2 et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract89

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Nanocomposites based on chitosan and montmorillonite for mesenchymal stem cells and regenerative cells transplantation

Natalia V. Smirnova1,3, Pavel V. Popryadukhin2, Vladimir E. Yudin2, Irina P. Dobrovolskaya2, Elena M. Ivankova2, Alexander B. Smolyaninov3

1Institute of Cytology, RAS, St. Petersburg, Russia; 2Institute of Macromolecular Compounds, RAS, St. Petersburg, Russia; 3Reseach laboratory of cell technologies of the North-Western State Medical University named after I.I. Mechnikov, "Stem Cell Bank Pokrovsky", St. Petersburg, Russia

Correspondence: Alexander B. Smolyaninov, "Stem Cell Bank Pokrovsky", Bolshoi Prospect Vasilevski Ostrov 85, St. Petersburg, 199106, Russia, E-mail: stemcellbank@spam is badinbox.ru

Abstract

This paper examines the structural and mechanical properties of composite materials based on chitosan and micro- and nanoparticles of Na-montmorillonite and the possibility of their application for the cultivation and targeted delivery of mesenchymal stem cells and regenerative cells.

Aim: One of the problems of tissue engineering is to develop a matrix for the proliferation of stem cells and regenerative cells. Materials based on chitosan can change the structure and properties in liquid media, making them unsuitable for the cultivation of cells. It can be proposed that the incorporation of Na-MMT particles in the chitosan matrix will help stabilize the structure and shape of the matrix, preserving the porous structure of the material in the liquid medium. On the other hand, an important characteristic of the materials used in cellular technologies is the lack of cytotoxicity, and support for effective cell adhesion, proliferation and differentiation.

Methods:
Composite films and sponges were prepared from chitosan (Sigma BioChemika, MM = 255kDa, degree of deacetylation 80%) filled with Na-MMT (Southern Clay Products, Inc., CEC = 92.6 mequiv/100g). Adipose tissue is collected aseptically via surgical liposuction. Adipose-derived stem cells (ASCs), were isolated from lipoaspirates in the Cell Technologies Laboratory, MNWMA. Dermal fibroblasts (DF) were isolated from human foreskin tissue using a standard dissociation protocol in Institute of Cytology, RAS. Adhesion and proliferation analyses of ASCs and DF cultivated on composites were carried out using immunostaining, MTT-test and SEM.

Results:
It’s been shown that by addition of Na-montmorillonite, biomaterial acquires structural and mechanical stability in the sterilization process and the handling of liquid media in cell culture. In vitro studies using DF and (ASCs) demonstrated that this material has a set of properties to ensure matrix biocompatibility and support of effective cell adhesion and proliferation.

Conclusions: Nanocomposites based on chitosan and Na-montmorillonite could be adapted for cells transplantation and tissue engineering applications.

Keywords:
biomaterial, chitosan, Na-montmorillonite, adipose derived stem cells, dermal fibroblasts, cell transplantation, tissue engineering