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ctt-journal > Smirnova N.1 et al. (Abstract)

Smirnova N.1 et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract88

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Telomere length in cord blood stem cells

Nataliya V. Smirnova, Anton A. Logachov, Tatiyana Y. Smirnova, Daniil A. Fateev,  Alexander B. Smolyaninov

Research laboratory of cell technologies of the North-Western State Medical University named after I. I. Mechnikov, St. Petersburg, Russia; "Stem Cell Bank Pokrovsky", St. Petersburg, Russia

Correspondence: Alexander B. Smolyaninov, "Stem Cell Bank Pokrovsky", Bolshoi Prospect Vasilevski Ostrov 85, St. Petersburg, 199106, Russia, E-mail: stemcellbank@spam is badinbox.ru

Abstract

Telomeres are the ends of the chromosomes and represent repeated DNA sequence and protein complexes, which form and stabilize the protective loop structure. This process allows the repa-ration system to distinguish the chromosomes ends from DNA double-breaks. Telomeres shorten with each cell division because of the “end replication problem”. This molecular mechanism limits the proliferative potential of cells.

Telomere length was measured by the flow-FISH method. We used special complementary te-lomere sequence peptide nucleic acid probes with FITC. Simultaneous DNA staining allows us to choose G0\G1 cells for analysis. Cell line 1301 (T-cell lymphoblastic leukemia) was used as the external control, because these cells have a stable telomere length.

Twenty cord blood samples were analyzed in this work. The average telomere length of the leu-kocytes fraction of the CB cells was 18.7±3.9 % when compared to the control cells. These re-sults completely correspond to literature data.

The proliferative activity of the stem cells, measured by the length of telomeres, may be an in-formative indicator of the quality of the graft. It is known that engraftment efficiency positively correlates with the telomere length of graft cells. This is additional evidence that transplanting cord blood is more effective than the transplantation of mobilized peripheral blood stem cells, as telomeres in the PC cells are longer. The umbilical cord blood cells underwent fewer cycles of division and have a replicative advantage over peripheral blood cells.

Keywords: telomere, stem cells, umbilical blood