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ctt-journal > Shaymardanova et al. (Abstract)

Shaymardanova et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract42

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Transplantation of human umbilical cord blood cells into the site of traumatic spinal cord injury of rats

Gulnara F. Shaymardanova1, Yana O. Muhamedshina2, Ilnur I. Salafutdinov3, Albert A. Rizvanov3, Yuriy A. Chelyshev2

1Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Science, Kazan, Russian Federation; 2Kazan State Medical University, Kazan, Russian Federation; 3Kazan (Volga Region) Federal University, Kazan, Russian Federation

Correspondence: Gulnara F. Shaymardanova, Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, 2/31, Lobachevsky st., 420111, Kazan, Russia, E-mail: gulnara_kzn@spam is badrambler.ru

Abstract

The use of stem cells offers new challenges in neurodegenerative disease therapy. It is known that umbilical cord blood cells (UCBCs) are a valuable source of hematopoietic and mesenchymal stem cells, endothelial, and other progenitor cells. They express various neurotrophic factors, and exert a modulation of stable functional and inflammatory reactions. Besides this, the effectiveness of combined gene and cell therapy was shown, for example, in brain ischemia.

Objective: To estimate the efficiency of transplantation of non-transfected UCBCs and UCBCs, transfected by double-cassette plasmid pBud-VEGF165-FGF2 expressing vegf and fgf2 genes; as well as the survival and migration ability of grafted UCBCs on spinal cord injury, using a rat model. UCBCs transfected with pBud-EGFP served as the control. It follows from morphometry data that at 30 days after transplantation both transfected and non-transfected UCBCs give rise to an increase in survival of gray and white matter at 3 and 5 mm rostral and caudal from the epicenter of trauma. Particularly, pBud-VEGF-FGF2 transfected UCBCs give rise to more than 60% enlargement of the area of preserved gray matter at 3 mm from the epicenter. A decrease in the area of pathological cavities and rising number of myelin fibers were observed at the external regions of white matter. It was revealed that grafted UCBCs survive in destructed tissue and migrate up to 10 mm in a rostral and caudal direction from the point of injection.

Keywords: umbilical cord blood cells, spinal cord, transfection, VEGF, FGF2