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ctt-journal > Ponomarev et al. (Abstract)

Ponomarev et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract38

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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The bone marrow of multiorgan donors and cadaveric tissue donors as an alternative source of hematopoietic stem cells

Ivan N. Ponomarev, Natalia V. Borovkova

Emergency Care Research Institute NV Sklifosovsky, Moscow, Russia

Correspondence: Ivan N. Ponomarev, Emergency Care Research Institute NV Sklifosovsky, B. Sukharevskaya sq., 3, Moscow, 129090, Russia, E-mail: rzam@spam is badyandex.ru

Abstract

The transplantation of bone marrow (BM) is a standard method of treatment in hematology and other fields of medicine. A deficiency of volunteer donors and the presence of contraindications for autotransplantation necessitates the search for an alternative source of hematopoietic stem cells (HSC). One of these potential sources is the BM of multiorgan donors and cadavers.

Aim: To evaluate the possibility of using BM of multiorgan donors and cadaveric tissue donors as an alternative source of hematopoietic stem cells.

Methods: The BM of 10 multiorgan donors and 30 cadaveric tissue donors was studied. BM was harvested by aspiration from the iliac bone in multiorgan donors before organ harvesting; in cadaveric tissue donors this was within 6 hours of the onset of biological death. The quantitative and functional usefulness of the BM was assessed by the number of CD45lowCD34+ cells, the binding of the vital dye 7AAD, and cell cultures.

Results: The concentration of HSC in the BM obtained from multiorgan donors amounted to 0.24 ± 0.06%, and cell viability was 91.4 ± 1.7%. In cadaveric tissue donors a BM content of 0.55 ± 8.4% HSC with a viability of 82.4 ± 2.5% was obtained. Hematopoietic colony formation was typical for BM cells obtained from volunteer donors.

Conclusions: The concentration, viability, and cultural properties of BM cells obtained from multiorgan donor and cadaveric tissue donors are comparable to the values of volunteer donors, and provides the possibility for use as an alternative source of HSCs.

Keywords: bone marrow, hematopoietic stem cells, multiorgan donor, cadaveric tissue donors