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ctt-journal > Lebedeva et al. (Abstract)

Lebedeva et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract21

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Distribution of HLA-haplotype frequencies in the population of the Moscow region

Lidia L. Lebedeva1,2, Tatiana V. Pukhlikova1,2, Tatiana A. Astrelina1,2, Maria V. Yakovleva1

1Stem Cell Bank, Moscow, Russia; 2Research Centre of Pediatric Hematology, Oncology and Immunology, Moscow, Russia

Correspondence: Lidia L. Lebedeva, Stem Cell Bank, Moscow, Russia, 31, Bakinskaya str., Moscow, 115541, Russia, E-mail: lebedeval@spam is badyahoo.com

Correspondence: Tatiana A. Astrelina, Stem Cell Bank, Moscow, Russia, 31, Bakinskaya street, Moscow, 115541, Russia, E-mail: t_astrelina@spam is badmail.ru

Abstract

Aim: To study the distribution of frequencies of HLA-haplotypes (2-locus (B/C and DRB1/DQB1), 4-locus (B/C/DRB1/DQB1) and 5-locus (A/B/C/DRB1/DQB1)) in the population of the Moscow region.

Methods: We investigated 200 samples of cord blood (CB), selected by independent randomization, and 400 samples of peripheral blood parents. Genotyping was performed by hybridization with oligonucleotide probes (SSO) and allele-specific primers (SSP). The frequency of haplotypes was determined by simply counting after the family segregation analysis.

Results: We found 50 variants of haplotype B/C (often found: B*35/C*04 (8.9%), B*08/C*07 (8.4%), B*13/*06C (8.4%), B*07/C*07 (7.9%), B*44/C*05 (5.6%) and B*18/C*12 (5.1%)); 15 variants of haplotype DRB1/DQB1 (often found: DRB1*11/DQB1*03 (14.4%), DRB1*04/DQB1*03 (13.0%), DRB1*07/DQB1*02 (13.0%), DRB1*01/DQB1*05 (11.1%), DRB1*15/DQB1*06 (11.1%), DRB1*13/DQB1*06 (10.2%) and DRB1*03/DQB1*04 (8.9%)); 112 variants on a 4-locus haplotype B/C/DRB1/DQB1 (often found: B*13/C*06/DRB1*07/DQB1*02 (8.45%), B*08/C*07/DRB1*03/DQB1*02 (7.46%), B*18/C*07/DRB1*11/DQB1*03 (3.48%), B*07/C*07/DRB1*15/DQB1*01 (3.48%), B*15/C*03/DRB1*04/DQB1*03 (3.01%)); 210 variants on a 5-locus haplotypes A/B/C/DRB1/DQB1 (often found: A*01/B*08/C*07/DRB1*03/DQB1*02 (5.05%), A*24/B*13/C*06/DRB1*07/DQB1*02 (2.27%), A*30/B*13/C*06/DRB1*07/DQB1*02 (1.8%), and A*02/B*15/C*03/DRB1*04/DQB1*03 (1.8%)).

Conclusions: For fast and efficient searching for compatible donor sample CB, the frequency distribution of HLA-haplotypes should be considered, as in the population of the Moscow region in general, it corresponds to other central-eastern European nations.

Keywords: HLA-haplotypes, cord blood, donor searching