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ctt-journal > Ivolgin D.2 et al. (Abstract)

Ivolgin D.2 et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract84

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Does the cord blood processing method affect the cord blood unit quality after thawing: improving cord blood banking procedures

Dmitry A. Ivolgin, Ksenia V. Korovina, Alexandrina S. Khrupina, Ksenia V. Shunkina, Alexander B. Smolyaninov

Research laboratory of cell technologies of the North-Western State Medical University named after I.I. Mechnikov, St. Petersburg, Russia; "Stem Cell Bank Pokrovsky", St. Petersburg, Russia

Correspondence: Alexander B. Smolyaninov, "Stem Cell Bank Pokrovsky", Bolshoi Prospect Vasilevski Ostrov 85, St. Petersburg, 199106, Russia, E-mail: stemcellbank@spam is badinbox.ru

Abstract

Aim: It has been widely considered that cord blood (CB) processing methods don’t affect the quality of the CB unit after it's thawed, but several cryobiological sources suppose that it does. The aim of the present study was to evaluate whether such a relationship exists.

Methods: A retrospective analysis of the parameters of 64 CB units, which were processed with automatic methods (Sepax S-100, Biosafe, Switzerland) (n=26) (Group 1), or manual methods (n=38) (Group 2), and cryopreserved and thawed in three different ways: the automatic method (Sepax S-100, Biosafe, Switzerland) (n=14), the NYBC method (n=21), and unwashed cord blood units (n=29). The recovery of nucleated cells was measured with a hematology analyzer ("ACT diff 2", Beckman Coulter, USA), and the recovery of CD34+/CD45+ cells and viability thereof were determined by flow cytometry (Cytomix FC500, Beckman Coulter, USA) in all groups.

Results: After thawing, the mean recovery of nuclear cells in Groups 1 and 2 was 98.2±3.06% and 87.8±2.86%, respectively; mean viability in Groups 1 and 2 was 90.5±3.98% and 85.2±2.8% respectively; and mean recovery of CD34/45+ in Groups 1 and 2 was 90.5±1.3% and, 89.8±1.19%, respectively. A similar correlation was observed when the subgroups of CB units in Group 1 that had been thawed by different methods were compared with those of Group 2. All results were statistically significant.

Conclusions:  Our data shows that the quality of thawed CB units that had been processed with the automated system was higher than those of CB units processed with manual methods. Therefore, our suggestion is that a CB processing method can influence its quality after thawing: but future researches and larger sample are needed to make definitive conclusion. In the situation when we have no standards for the methods of CB processing this data can serve as an additional argument for automated CB stem cell separation as the method of choice in CB banking in our country. 

Keywords: cord blood, cryopreservation, Sepax S-100, processing, thawing of cord blood