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ctt-journal > Isaeva et al. (Abstract)

Isaeva et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract52

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Laboratory signs of T-cell anergy in cases of B-cell chronic lymphocytic leukemia

Natalia V. Isaeva, Galina A. Zaitseva, Tamara P. Zagoskina, Yelena A. Poponina

Federal State Establishment "Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical-Biological Agency", Kirov, Russia

Correspondence: Natalia V. Isaeva, FSE "Kirov Research Institute of Hematology and Blood Transfusion of the Federal Medical-Biological Agency", 72, Krasnoarmeyskaya str., Kirov, 610027, Russia, E-mail: immunlab@spam is badyandex.ru

Abstract

The aim of the study was to analyze the expression levels of the main signaling molecules by T-lymphocytes, their subpopulations, and NK-cells in patients with newly diagnosed B-cell chronic lymphocytic leukemia (CLL).

The geometric mean of fluorescence intensity of the markers CD45, CD3, CD4 and CD8 on the blood lymphocytes were determined using flow cytometry in 60 patients at the diagnosis stage of CLL.

These patients showed a significant decrease of the intensity of expression of CD45 and CD3 within the whole population of T-lymphocytes and CD4 within T-lymphocytes/helpers, when compared with the same indices of the control group (p <0.001 for each). No abnormalities were detected in the indicators of the CD8-expression intensity on cytotoxic T-lymphocytes and NK-cells ("serial killers"). Normal or increased number of T-lymphocytes, their main subpopulations, and NK-cells in peripheral blood were significantly frequent in patients with manifestations of CLL, which can be explained by the action of some cytokines, synthesized by tumor cells, on cell proliferation.

Thus, some patients in CLL manifestation have signs of T-lymphocytes anergy, in particular with helper properties. Probably, these lymphocytes undergo constant activation in the absence of co-stimulatory signals. In contrast, a similar trend for CD8+-lymphocytes was not established. These results indicate the strengthening of passive tolerance to autoantigens, tenseness of antitumor immunity, and a weakening of the anti-infection defense.

Keywords: chronic lymphocytic leukemia, anergy of lymphocytes, T-lymphocytes, NK-cells