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ctt-journal > Dubrava et al. (Abstract)

Dubrava et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract09

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting Hematopoietic Stem Cell Transplantation in Children and Adults", Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Assessment of the genetic profile of fetal liver stem compartment cells after cryopreservation — prerequisites for effectiveness of application in the therapy of autoimmune disorders

Tatyana G. Dubrava, Elena D. Lutsenko, Yekaterina Ye. Yampolskaya, Lyudmila V. Ostankova, Maxim V. Ostankov, Anatoliy N. Goltsev

Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, Kharkov, Ukraine

Correspondence: Tatyana G. Dubrava, Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, 23 Pereyaslavskaya Str., 61015 Kharkov, Ukraine, E-mail: cryopato@spam is badrambler.ru

Abstract

Imbalance in the structural and functional organization of immune and hemopoietic systems during autoimmune disorders determines a necessity for the search of preparations able to correct their state. They may include fetal liver cells (FLCs) containing hemopoietic (HSCs) and mesenchymal (MSCs) stem cell elements. Using cryobiotechnologies, the team set out to comprehensively assess the qualitative characteristics of FLC, including assessment at the molecular level.

Aim: A comparative assessment of expression of genes regulating hemopoietic and immunomodulating activity in HSCs and MSCs of cryopreserved FL of different gestation terms.

Methods: The objects of study were CBA/H mice FLCs at the 14th and 18th days of gestation. Cryopreservation was performed according to the regimen proposed by Goltsev A.N. (1995). Gata-2 and ido gene expression was determined with use of PCR-technologies on the bioanalyzer “Agilent 2100” (USA).

Results: It was shown that the same cryopreservation regimen can activate or inhibit in HSCs and MSCs gata2 and ido gene expression depending on the FL gestation term. Cryopreservation increased expression levels of the studied genes in FL at late gestation terms.

Conclusions: An established fact of the high expression level of gata2 and ido genes in cryopreserved FLCs at the 18th gestation day make it possible to assume that they have a high therapeutic potential due to their dualistic hemopoietic and immunocorrective activity.

Keywords: hemopoietic stem cells, fetal liver, cryopreservation