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ctt-journal > Baranova et al. (Abstract)

Baranova et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 3, No. 12
doi: 10.3205/ctt-2011-No12-abstract01

© The Authors. This abstract is provided under the following license: Creative Commons Attribution 3.0 Unported

Abstract accepted for "5th Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation",
Saint Petersburg, Russia, September 18–20, 2011

Preliminary Program

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Autograft cell content and cytokine production in apheresis products collected using different forms of G-CSF in patients with hematological malignancies

Dariya S. Baranova, Irina V. Kruchkova, Nataliya V. Pronkina, Egor V. Batorov, Marina A. Tihonova, Аndrey V. Gilevich, Vera V. Sergeevicheva, Svetlana А. Sizikova, Galina Yu. Ushakova, Tatiyana I. Pospelova, Alexander A. Ostanin, Elena R. Chernykh

Research Institute of Clinical Immunology SB RAMS, Novosibirsk State Medical University, Novosibirsk, Russia

Correspondence: Dariya S. Baranova, Research Institute of Clinical Immunology SB RAMS, 630099, 14, Yadrintsevskaya str., Novosibirsk, Russia, E-mail: bmt-novosibirsk@spam is badmail.ru

Abstract

Aim: To characterize the features of apheresis product cell subtypes' contents and their functional activity, which were collected using different forms of G-CSF in mobilization regimens.

Methods:
Twenty-four malignant lymphoma patients who underwent HSCT were included in the study. HSCs were mobilized using standard protocols of chemotherapy combined with either G-CSF (Filgrastim; 5 μg/kg/day; n=15) or pegylated G-CSF (Neulastim; 6 mg, single injection; n=9). Lymphocyte subtypes were evaluated using flow cytometry. Cytokine production in autograft cell cultures was analyzed by flow fluorometry multiplex assay.

Results: Compared to filgrastim apheresis, products that were collected using pegfilgrastim, contained a higher absolute lymphocyte amount (including CD3+, CD4+CDRO+, CD19+, CD4+CD25high and CD4+CD25+CD127- T-cells) and a lower granulocyte amount. Also there were higher levels of IL-2, IL-7, IL-10 production and a lower secretion of IL-6 and IL-8 in the pegfilgrastim group. The times of leukocyte engraftment and absolute lymphocyte count on day +15 after HSCT were not significantly different in both groups.

Conclusions: Apheresis product collected using pegylated G-CSF has quantitative and qualitative differences which do not significantly influence the efficiency of early lymphocyte recovery.

Keywords: apheresis product, autologous hematopoietic stem cell transplantation, filgrastim, pegfilgrastim