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ctt-journal > Mamaev et al. (Abstract)

Mamaev et al. (Abstract)

Cellular Therapy and Transplantation (CTT), Vol. 2, No. 5, 2009
doi: 10.3205/ctt-2009-No5-abstract07
© The Authors. This abstract is provided under the following license:
Creative Commons Attribution 3.0 Unported


Abstract accepted for "Joint EBMT Pediatric Working Party – 3rd Raisa Gorbacheva Memorial Meeting on Hematopoietic Stem Cell Transplantation", Saint Petersburg, Russia, September 17–20, 2009

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Posttransplantation leukemia relapses: the role of chromosome changes

Nikolay N. Mamaev, Tatyana L. Gindina, Ildar M. Barkhatov, Boris V. Afanasyev

Memorial R.M. Gorbacheva Institute of Children Hematology and Transplantation, St. Petersburg Pavlov State Medical University, St. Petersburg, Russia

Correspondence: Nikolay Mamaev, Memorial R.M. Gorbacheva Institute of Children Hematology and Transplantation, St. Petersburg Pavlov State Medical University, 6/8, Tolstoy str., St. Petersburg, 199044, Russia, E-mail: nikmamaev@spam is badrambler.ru

Abstract

Aim: To study the cytogenetic profile of leukemia cells in patients with relapses after hematopoietic stem cell transplantation (HSCT).

Patients and methods: The group enrolled 5 patients with acute myeloid (3) and acute lymphoblastic (2) leukemias. The standard G-banding cytogenetic technique supplemented by rtPCR was used.

Table 1. Karyotypes in patients with relapsed leukemias after HSCT

Disease Karyotype
ALL 46,XX, t(9;11)(p22;q23) (100%)
ALL 45,XX, der(2)t(2;9)(p21;q13), t(4;12)(q25;q13), -9, t(11;14)(p13;q11), del(17)(p11) (100%)
ALL 47,XX, dic(6;11)(q23;?), del(9)(q13), t(9;22)(q34;q11), del(12)(q13), -13, +der(22)t(9;22)(q34;q11), +22 (30%)
AМL 44,XX, der(1)t(1;18)(p10;p10), del(3)(p13), der(4)t(4?;11) (q35;?;q?13), -5, der(6)t(1;6)(p10;q10), del(7)(q22), +del(8)(p21), add(12)(q12), -17, -18, -19, add(20)(q13), +22(80%)/46,XY(20%)
AМL 50,XY, +4, +8, +10, +22(50%)/47,XY, +8(40%)/46,XY(10%)


Results:
Modal classes of chromosomes ranged from 44 up to 50 (Table). Four patients were associated with multiple chromosome changes and 1 with translocation (9;11)(p22;q23). Of the 5 tested patients, 3 (60%) contained the whole or partial loss of chromosome number 9. Several patients lost chromosomes 13 and 17, while they gained chromosome number 8. The following structural chromosome rearrangements are important: a) translocations (1;6)(p10;q10), (1;18)(p10;p10), (4;12)(q25;q13), and (11;14)(p13;q11); and b) deletions of 7q22 and 3p. 

Conclusion: Multiple chromosome changes and the loss of 9/9p- are the most common findings in patients with posttransplantation relapse.

Keywords: leukemia, hematopoietic stem cell transplantation, relapse, chromosome changes

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