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Ocheni et al.

Cellular Therapy and Transplantation (CTT), Vol. 1, No. 3

Please cite this article as follows: Ocheni S, Schafhausen P, Bacher U, Fehse B, Kröger N. Syngeneic Graft-Versus-Chronic-Myeloid-Leukemia-Effect? Cell Ther Transplant. 2009;1:e.000031.01. doi:10.3205/ctt-2009-en-000031.01

© The Authors. This article is provided under the following license: Creative Commons Attribution 3.0 Unported
Submitted: 30 October 2008, accepted: 15 January 2009, published: 18 May 2009


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Syngeneic Graft-Versus-Chronic-Myeloid-Leukemia-Effect?

Sunday Ocheni1, Philippe Schafhausen2, Ulrike Bacher1, Boris Fehse1, Nicolaus Kröger1

1Dept. for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Germany; 2Department of Oncology and Hematology, University Cancer Center Hamburg, Hamburg, Germany

Correspondence: Prof. Dr. med. Nicolaus Kröger, Dept. for Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Martinistrasse 52, D-20246 Hamburg, Germany, Tel.: +49-40-74105-5864, Fax: +49-40-74105-3795, E-mail: nkroeger@spam is baduke.uni-hamburg.de

Summary

Fefer et al first suggested in 1979 that the Ph1-positive clone in chronic myeloid leukemia (CML) can be eradicated by chemotherapy, and that the marrow can be repopulated by stem cells from identical twins. Since then, there have been increasing reports of a graft-versus-leukemia-like effect following syngeneic stem cell transplantation (SCT) in CML. This case report describes three CML patients who achieved complete hematological, cytogenetic and molecular remission following syngeneic SCT. We suggest that syngeneic SCT should be included in the treatment decisions for CML patients with available identical twins, considering the reduced incidence of morbidity and mortality compared to allogeneic SCT.

Keywords: syngeneic stem cell transplantation, chronic myeloid leukemia, graft versus leukemia effect

Report

Chronic myeloid leukemia (CML) is a malignant disorder of hematopoiesis resulting from the clonal expansion of a primitive hematopoietic cell that, for a variable period of time, retains the capacity to differentiate, leading to marked marrow hyperplasia and increased numbers of myeloid cells and platelets in the peripheral blood [1]. The hallmark of this disorder is the Philadelphia (Ph) chromosome genetic abnormality t (9; 22) (q34; q11) in which there is a reciprocal translocation between the long arms of chromosomes 9 and 22 resulting in the BCR-ABL fusion, and it is present in about 90% of patients with CML [2]. There have been several treatment strategies for this disorder including busulfan, hydroxyurea, and alpha interferon (α-IFN), resulting in varying degrees of hematological and cytogenetic remission. The introduction of the tyrosine kinase inhibitor imatinib had a major impact and brought a dramatic change to the management of CML. It induces a high number of complete cytogenetic remissions, but molecular remission is rarely observed. While imatinib is a highly effective therapy for CML, both de novo and acquired resistance have been observed. In those cases where it has been studied, resistance has been associated with BCR-ABL gene amplification in some, and mutations in BCR-ABL that prevent imatinib from inhibiting the kinase in others [1,3]. In some patients, point mutations in BCR-ABL can be found at diagnosis and, with imatinib treatment, cells bearing these mutations may undergo positive selection [1,4]. The long-term benefits of imatinib are currently unknown and although efforts should be made to control the problem of resistance [5], alternative cure strategies have to be developed.

Currently, allogeneic stem cell transplantation (allo-SCT) remains the only curative treatment approach, but due to its high treatment-related morbidity and mortality, the timing of allo-SCT in the “tyrosine kinase era” remains to be determined. Allo-SCT is currently reserved for poor responders to imatinib and its derivatives as well as high-risk patients [6]. The curative potential of allo-SCT in leukemias has been demonstrated from various clinical data to be partly due to its graft-versus-leukemia (GvL) effect. Currently, very little data exists to support the concept of GvL effect in syngeneic SCT. The pioneers in the use of syngeneic SCT in CML have been Dr. A. Fefer and his colleagues of the Seattle Marrow Transplant Team at the Fred Hutchinson Cancer Research Center, University of Washington. In 1979 [7], they published the results of identical twin transplants in four patients with chronic phase CML treated with dimethyl busulfan, cyclophosphamide (CY), and a single 920 rads exposure of total body irradiation (TBI). All four recovered with Ph-negative normal hematopoiesis. The same group also reported in 1982 [8] on twelve patients in the chronic phase of Ph1 (Philadelphia)-positive chronic granulocytic leukemia (CGL) who received chemoradiotherapy and marrow from their healthy identical twins. All achieved complete remission, with disappearance of all Ph1-positive cells. In 1986 [9], they published another report of syngeneic marrow transplantation in hematological cancers including sixteen patients who received transplantation in the chronic phase of Ph1+ chronic granulocytic leukemia (CGL). All showed disappearance of all Ph1+ cells. Other authors who have shown that the Ph1-positive clone in CML can be eradicated by chemotherapy and the marrow repopulated by stem cells from normal twins include Mackinnon et al [10], Fujii et al [11], Littleton et al [12] and Pelosini et al [13].

Several groups such as the European LeukemiaNet have developed a treatment algorithm in the management of chronic myeloid leukemia [14]. To this detailed treatment algorithm we would like to add the possibility of syngeneic stem cell transplantation in patients with chronic myeloid leukemia (CML) if an identical twin is available. This constellation occurs rarely—in about 1 out of 300 patients—but it offers a curative therapeutic option with low treatment-related mortality.

We report on three patients with a median age of 42 years [range 25 to 58] who were transplanted from an identical twin between 1997 and 2001 at the University Hospital Hamburg/Germany. All patients were at the chronic phase and had received pre-treatment with hydroxyurea with or without interferon. At time of transplantation all patients still had Philadelphia chromosome positive metaphase and detectable BCR-ABL transcripts. In two of the patients the conditioning regimen consisted of an intensified regimen with total body irradiation 12 Gray, Busulfan 8 mg/kg, and Cyclophosphamid 60 mg/kg, while the third patient was conditioned with Busulfan [14 mg/kg] and Cyclophosphamid [120 mg/kg]. All patients received peripheral blood stem cells with a median dose of 4.3 x 106 CD34+ cells/kg (range 3.5–8.2) The toxicity consisted mainly of mucositis grade II according to the Bearman scale. There was a rapid engraftment of the leukocytes >1.0/nl at a median of 9 days [range 8–10]. Two patients developed a mild maculopapulous exanthem of the skin, which was verified by histological examination as acute graft-versus-host-disease.

With a median follow-up of 5 years [range 4–7.5], all patients are in complete hematological, cytogenetic, and molecular remission [table 1]. Molecular remission was determined by highly sensitive methods as described recently [15]. This long lasting molecular remission suggests a syngeneic graft-versus-chronic-myeloid-leukemia-effect, since in the German CML study none of the 23 patients who received autologous transplantation achieved a molecular remission during follow-up. The results in these patients support other published data [6-13] in which a GvL effect has been induced in syngeneic SCT in CML patients. The possible targets for the syngeneic graft-versus-leukemia effect in CML might be proteinase 3 or PRAME and to a lesser extent BCR/ABL transcript [16]. The observation of mild acute graft-versus-host disease of the skin after syngeneic stem cell transplantation has been reported by others [13,17,18]. In the case of Pelosini et al [13], their patient has remained in complete hematological and molecular remission and in good clinical condition three years after syngeneic SCT. In a syngeneic mice model it has been shown that interleukin-2 and Cyclosporin after twin transplantation reduces the relapse rate via graft-versus-leukemia effect without graft-versus-host disease [19].

Table 1. Patients' characteristics

  Gender Age Conditioning Engraftment > 1,0 /nl Acute GvHD bcr/abl
1. m 25 TBI 12 Gy Bu 8 mg/kg Cy 60 mg/kg day+9 skin I negative 7,5 years +
2. m 42 TBI 12 Gy Bu 8 mg/kg Cy 60 mg/kg day+8 Ø negative 4 years +
3. f 58 Bu 14 mg/kg Cy 120 mg/kg day+10 skin I negative 5 years +


The pathophysiological mechanisms of graft-versus-host-disease (GvHD)-like syndromes in syngeneic SCT in humans are yet to be fully understood. In 2003, Latif et al [20] summarized all 17 cases of severe GvHD previously reported in the literature following syngeneic SCT in addition to detailed reports of their own two patients. Postulated mechanisms include mediation by auto reactive lymphocytes directed at MHC class II proteins and the use of cyclosporin A [21]. The role of cyclosporin A (CsA) itself is poorly understood. The fact that some of the patients did not receive CsA means that other mechanisms may exist which should be further studied [20]. Another possibility that might contribute to the lower risk of relapse in comparison to autologous stem cell transplantation is the fact that the stem cell graft from a syngeneic donor is not contaminated with CML cells.

In conclusion, we suggest that if an identical twin is available, syngeneic stem cell transplantation should be included in the treatment decisions in CML since it has a lower treatment-related mortality than allogeneic stem cell transplantation, taking into account the increasing reports of a GvL-like syndromes following syngeneic SCT in this disorder, and the fact that it induces long-lasting molecular remission in contrast to imatinib mesylate. Further studies should be carried out to determine the mechanisms that promote GvHD-like effects in recipients of syngeneic stem cell transplantation.

References

[References with links indicate that an article is available Open Access]

1. Appelbaum FR. Allogeneic transplantation for chronic myeloid leukaemia. In: Blume KG, Forman SJ, Appelbaum FR (eds) Thomas’ Hematopoietic cell transplantation. Blackwell Publishing, 3rd Ed. Ch.73. 2004;1007-1017.

3. Gorre ME, Sawyers CL. Molecular mechanisms of resistance to STI571 in chronic myeloid leukaemia. Curr Opin Hematol. 2002;9:303-307.

5. O’Hare T, Corbin AS, Druker BJ. Targeted CML therapy: controlling drug resistance, seeking cure. Curr Opin Genet Dev. 2006;16(1):92-99.

6. Bornhauser M, Kroger N, Schwerdtfger R, Shafer-Eckart K, Sayer HG, Zander AR. Allogeneic haematopoietic cell transplantation for chronic myelogenous leukaemia in the era of imatinib: a retrospective multicentre study. Eur J Haematol. 2006;76:9-17.

7. Fefer A, Cheever MA, Thomas ED et al. Disappearance of Ph1-positive cells in four patients with chronic granulocytic leukemia after chemotherapy, irradiation and marrow transplantation from an identical twin. N Engl J Med. 1979;300(7):333-337.

8. Fefer A, Cheever MA, Greenberg PD et al. Treatment of chronic granulocytic leukemia with chemoradiotherapy and transplantation of marrow from identical twins. N Eng J Med. 1982;306(2):63-68.

9. Fefer A, Cheever MA, Greeberg PD. Identical-twin (syngeneic) marrow transplantation for hematologic cancers. J Natl Caner Inst. 1986;76(6):1269-1273.

10. Mackinnon S, Hows JM, Goldman JM. Induction of a syngeneic graft-versus-leukemia effect following bone marrow transplantation for chronic myeloid leukemia. Leukemia. 1990;4(4):287-291.

11. Fujii H, Ueda Y, Nakagawa H, Sasai Y, Horiike S, Taniwaki M. Syngeneic peripheral blood stem cell transplantation for chronic myelogenous leukaemia associated with Klinefelter’s syndrome. Rinsho Ketsueki. 1999;40(3):218-223.

12. Littleton R, Penza SL, Avalos BR, Copelan EA. Syngeneic vs. allotransplantation in chronic myeloid leukaemia: all’s well that ends well. Transplantation. 2005;80(3):426-427.

13. Pelosini M, Galimberti S, Benedetti E, et al. Skin and stomach graft versus host disease after syngeneic BMT in CML: a case report.  Leuk Res. 2007. doi:10.1016/j.leukres.2006.12.018.

17. Rappeport J, Mihm M, Reinherz E, et al. Acute graft-versus-host disease in recipients of bone marrow transplants from identical twin donors. Lancet. 1979;2:717-720.

18. Einsele H, Ehninger G, Schneider EM, et al. High frequency of graft–versus-host like syndromes following syngeneic bone marrow transplantation. Transplantation. 1988;45:579-585.

© The Authors. This article is provided under the following license: Creative Commons Attribution 3.0 Unported

Please cite this article as follows: Ocheni S, Schafhausen P, Bacher U, Fehse B, Kröger N. Syngeneic Graft-Versus-Chronic-Myeloid-Leukemia-Effect? Cell Ther Transplant. 2009;1:e.000031.01. doi:10.3205/ctt-2009-en-000031.01

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