Lange et al. abstract

Please cite this article as follows: Lange C, Cakiroglu F, Spiess A, Cappallo-Obermann H, Zander AR. Platelet lysate for rapid expansion of human mesenchymal stromal cells. Cell Ther Transplant. 2008;2:e.000016.01. doi:10.3205/ctt-2008-en-000016.01

Platelet lysate for rapid expansion of human mesenchymal stromal cells

Claudia Lange, Figen Cakiroglu, Andrej Spiess, Heike Cappallo-Obermann, Axel R. Zander

Abstract

Human bone marrow mesenchymal stromal cells (hMSC) are promising candidates for new treatment options in transplant and regenerative medicine. However, most expansion protocols still use fetal calf serum (FCS) as growth factor supplement, which is a potential source of undesirable xenogeneic pathogens. We established an easy and reproducible expansion protocol for hMSC based on the addition of platelet lysate (PL) obtained from human thrombocyte concentrates. Both CFU-F and cumulative cell numbers were significantly increased compared to the conventional FCS-based medium. The generated cells meet all criteria for MSCs, e.g. plastic adherence, spindle-shaped morphology, surface marker expression, lack of hematopoietic markers, and differentiation capability into 3 mesenchymal lineages. Human MSC expanded with PL revealed favorable immunological properties in vitro. Gene expression profiles showed upregulation of cell cycle and DNA replication genes and downregulation of developmental, differentiation, adipogenic and MHC II genes. Thus, PL provides a safe component for accelerated and safe hMSC expansion.

Keywords: mesenchymal stromal cells, platelet lysate, gene expression

Please cite this article as follows: Lange C, Cakiroglu F, Spiess A, Cappallo-Obermann H, Zander AR. Platelet lysate for rapid expansion of human mesenchymal stromal cells. Cell Ther Transplant. 2008;2:e.000016.01. doi:10.3205/ctt-2008-en-000016.01

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